Session 10 – Gene Editing & Microbiome
Date: 26 July (Friday) 14:00 – 18:00 Venue: 3F, Bldg. A, CTBC Financial Park, Ballroom B
You-Tzung (Bob) Chen
National Taiwan University College of Medicine
Prof. Bob Chen got his bachelor- and master-trainings in National Taiwan University. His Ph.D. training was performed under the guidance of Prof. Allan Bradley, a pioneer in using transgenic mouse models for drug discovery, in Baylor College of Medicine. After a 5.5-year postdoctoral training at The University of Texas MD Anderson Cancer Center, Prof. Chen came back to Taiwan as a faculty and he is currently affiliated with the Graduate Institute of Medical Genomics and Proteomics at National Taiwan University College of Medicine. His research focus has been associated with the development of mouse genetics tools and recently with an emphasis on the CRISPR/Cas9-mediated somatic mutagenesis approach to generate cancer mouse models. Session Speech Title & Synopsis: Mouse Germline and Somatic Mutants Generated by CRISPR/Cas9 for Cancer Studies
Sophisticated genetic engineering techniques developed since the establishment of mouse embryonic stem cell culture have made mouse the model for biomedical research and drug discovery. The application of the CRISPR/Cas9 system for eukaryotic genome editing since 2013 further gave the opprotunity to lower the cost and shorten the time required for generating mutant mice to model human diseases. Here I will present a transgenic inducible Cas9 effector/CRISPR mutagen (ICE CRIM) mouse model as a somatic mutagenesis tool to study the corresponding human cancers. The ICE CRIM mouse model potentially offered a more efficient option to perform in situ functional studies on cancer gene mutant allelic series as well as their combinatorial effects.